Kilár F, Fanali S
Istituto di Cromatografia, CNR, Area della Ricerca di Roma, Monterotondo Scalo.
Electrophoresis. 1995 Aug;16(8):1510-8. doi: 10.1002/elps.11501601250.
Enantiomers can be separated by using human serum transferrin as a chiral phase. With the help of the native protein we were able to separate enantiomers with high efficiency, using a low ionic strength 2-(N-morpholino)ethanesulfonic acid (MES) buffer, pH 6, in capillary zone electrophoresis. Tryptophan methyl, ethyl and butyl ester enantiomers-moving towards the cathode at pH 6-were resolved by passing through an iron-free transferrin zone in coated capillaries. Since the isoelectric point of the iron-free transferrin is a little higher than 6, the protein zone is either not moving in the experiment or is slowly moving towards the anode. Under the simplest experimental conditions the highest resolution was obtained for the butyl ester enantiomers and the lowest for the methyl ester ones. By changing the experimental conditions, however, this order could be reversed. The results indicate that the lengths of the alkyl chains in the enantiomers have a significant effect on the resolution, i.e., on the interaction between the protein and the separands.
可以使用人血清转铁蛋白作为手性相来分离对映体。借助天然蛋白质，我们能够在毛细管区带电泳中，使用低离子强度的pH 6的2-(N-吗啉代)乙磺酸(MES)缓冲液高效分离对映体。色氨酸甲酯、乙酯和丁酯对映体在pH 6时向阴极移动，通过涂层毛细管中的无铁转铁蛋白区得以分离。由于无铁转铁蛋白的等电点略高于6，在实验中蛋白质区要么不移动，要么缓慢向阳极移动。在最简单的实验条件下，丁酯对映体的分辨率最高，甲酯对映体的分辨率最低。然而，通过改变实验条件，这个顺序可能会颠倒。结果表明，对映体中烷基链的长度对分辨率有显著影响，即对蛋白质与被分离物之间的相互作用有显著影响。
