Schmid M G, Gübitz G, Kilár F
Institute of Pharmaceutical Chemistry, Karl-Franzens University of Graz, Austria.
Electrophoresis. 1998 Feb;19(2):282-7. doi: 10.1002/elps.1150190223.
Further studies on chiral resolution of drugs with different chemical structures by capillary zone electrophoresis using iron-free human serum transferrin are described. The substances passed a highly concentrated pseudo-stationary protein zone applied in a coated capillary and the possible chiral separation of the optical isomers was followed. Eighteen drugs with different structures were screened, and the enantiomers of clofedanol, buphenine, acebutolol and chlorphenamine were resolved. Several, but not all drugs, showed longer migration times while passing the protein zone, indicating an interaction with transferrin, although chiral resolution was not observed in all cases. The observations provided further information about the properties of the surface interaction sites of transferrin.
本文描述了使用无铁人血清转铁蛋白通过毛细管区带电泳对不同化学结构药物进行手性拆分的进一步研究。这些物质通过涂覆毛细管中施加的高浓度假固定蛋白区，并跟踪光学异构体可能的手性分离。筛选了18种结构不同的药物，氯苯达诺、布非宁、醋丁洛尔和氯苯那敏的对映体得到了拆分。几种（但不是所有）药物在通过蛋白区时迁移时间延长，表明与转铁蛋白有相互作用，尽管并非在所有情况下都观察到手性拆分。这些观察结果提供了关于转铁蛋白表面相互作用位点性质的更多信息。
