Coppola L, Giunta R, Verrazzo G, Luongo C, Sammartino A, Vicario C, Giugliano D
Department of Gerontology, Geriatrics and Metabolic Diseases, Second University of Naples, Italy.
Diabete Metab. 1995 Oct;21(4):252-5.
The use of ozone in the treatment of peripheral vascular disease (PVD) is increasing. The purpose of this study was to evaluate the effect of ozone on haemoglobin oxygen affinity in Type-2 diabetic patients with PVD. Twenty diabetic patients presenting with PVD (Clinical stage II-IV according to Fontaine) and 20 non-diabetic healthy matched subjects were studied. In both groups, aliquots of blood were ozonised with mixtures of oxygen-ozone (O2-O3) to reach end-concentrations of 6.5, 13, 26 and 78 micrograms O3 per ml of substrate. At baseline, diabetic patients presented significantly lower haemoglobin oxygen affinity values but higher plasma levels of free haemoglobin and malonyldialdehyde (MDA) than controls. In both diabetic patients and controls, exposure of blood to ozone reduced haemoglobin oxygen affinity in an "all-or-none" fashion, without changing 2-3, diphosphoglycerate concentrations in erythrocytes. Both free haemoglobin and MDA concentrations showed significant, dose-dependent increases after blood ozonisation. Thus, ozone caused a significant increase in oxygen unloading of haemoglobin in both normal subjects and Type-2 diabetic patients with PVD.
臭氧在周围血管疾病（PVD）治疗中的应用正在增加。本研究的目的是评估臭氧对2型糖尿病合并PVD患者血红蛋白氧亲和力的影响。研究了20例患有PVD的糖尿病患者（根据Fontaine分级为临床II-IV期）和20名年龄、性别匹配的非糖尿病健康受试者。两组均将血液样本与氧-臭氧（O2-O3）混合物进行臭氧化处理，使底物中臭氧的终浓度分别达到6.5、13、26和78微克/毫升。基线时，糖尿病患者的血红蛋白氧亲和力值显著低于对照组，但血浆游离血红蛋白和丙二醛（MDA）水平高于对照组。在糖尿病患者和对照组中，血液暴露于臭氧后均以“全或无”的方式降低了血红蛋白氧亲和力，且红细胞内2,3-二磷酸甘油酸浓度未发生变化。血液臭氧化后，游离血红蛋白和MDA浓度均呈显著的剂量依赖性增加。因此，臭氧使正常受试者和2型糖尿病合并PVD患者的血红蛋白氧卸载显著增加。
