Perlin M W, Duggan D J, Davis K, Farr J E, Findler R B, Higgins M J, Nowak N J, Evans G A, Qin S, Zhang J
Computer Science Department, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA.
Genomics. 1995 Jul 20;28(2):315-27. doi: 10.1006/geno.1995.1148.
Inner product mapping (IPM) has been proposed as a hybridization-based method for achieving low-cost, high-throughput, high-resolution radiation hybrid (RH) mapping of clones. Using Alu-PCR products of chromosome 11-specific clones, we serially hybridized a set of RHs against gridded filters of YACs having an average size of 350 kb. We then combined these hybridization data with preexisting RH map data to build an inner product map. This binning of 865 YACs provides the first high-resolution large-scale (> twofold redundancy) clonal coverage of human chromosome 11 and is the first inner product map ever constructed. We verified the accuracy and precision of this chromosome 11 map by performing a novel likelihood analysis on independent YAC hybridization data. These results establish that IPM is a highly rapid, inexpensive, accurate, and precise large-scale long-range mapping method, particularly when preexisting RH maps are available, and that IPM can replace or complement more conventional short-range mapping methods. IPM may enable the rapid construction of sequence-ready maps and the binning of expressed sequences.
内积映射（IPM）已被提出作为一种基于杂交的方法，用于实现克隆的低成本、高通量、高分辨率辐射杂种（RH）作图。利用11号染色体特异性克隆的Alu-PCR产物，我们将一组RH连续与平均大小为350 kb的YAC网格滤膜杂交。然后，我们将这些杂交数据与现有的RH图谱数据相结合，构建了一个内积图谱。对865个YAC进行的这种分箱提供了人类11号染色体首个高分辨率大规模（>两倍冗余）的克隆覆盖，并且是有史以来构建的首个内积图谱。我们通过对独立的YAC杂交数据进行新颖的似然分析，验证了这个11号染色体图谱的准确性和精确性。这些结果表明，IPM是一种高度快速、廉价、准确且精确的大规模长程作图方法，特别是在有现有的RH图谱时，并且IPM可以替代或补充更传统的短程作图方法。IPM可能有助于快速构建序列就绪图谱以及对表达序列进行分箱。
