Kumlien J, Grigoriev A, Roest Crollius H, Ross M, Goodfellow P N, Lehrach H
Department of Genome Analysis, Imperial Cancer Research Fund, P.O. Box 123, 44 Lincoln's Inn Fields, London WC2A 3 PX, UK.
Mamm Genome. 1996 Oct;7(10):758-66. doi: 10.1007/s003359900227.
We present a radiation hybrid (RH) map of human Chromosome (Chr) X, using 50 markers on 72 radiation hybrids. The markers, obtained from the consensus map, form a grid spanning the entire chromosome. To check the RH map, the marker order was determined by analysis of presence or absence of retained human DNA fragments in the RHs; the comparison with the consensus showed a similar order. Any STSs, microsatellites, genes, and clones can be positioned and ordered relative to the marker grid. This approach integrates genetic, physical, and large-scale clone mapping and is used to link YAC contigs containing data from various experimental sources.
我们利用72个辐射杂种细胞上的50个标记构建了人类X染色体的辐射杂种（RH）图谱。这些标记取自一致性图谱，形成了覆盖整条染色体的网格。为检验该RH图谱，通过分析辐射杂种细胞中保留的人类DNA片段的有无来确定标记顺序；与一致性图谱的比较显示顺序相似。任何序列标签位点（STS）、微卫星、基因和克隆都可相对于标记网格进行定位和排序。这种方法整合了遗传、物理和大规模克隆图谱，用于连接包含来自各种实验来源数据的酵母人工染色体（YAC）重叠群。
