Hartzog G A, Basrai M A, Ricupero-Hovasse S L, Hieter P, Winston F
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
Mol Cell Biol. 1996 Jun;16(6):2848-56. doi: 10.1128/MCB.16.6.2848.
Spt4p is a nonhistone protein of Saccharomyces cerevisiae that is believed to be required for normal chromatin structure and transcription. In this work we describe the isolation and analysis of a human gene, SUPT4H, that encodes a predicted protein 42% identical to Spt4p. When expressed in S. cerevisiae, SUPT4H complemented all spt4 mutant phenotypes. In human cells SUPT4H encodes a nuclear protein that is expressed in all tissues tested. In addition, hybridization analyses suggest that an SUPT4H-related gene is also present in mice. SUPT4H was localized to human chromosome 17 by PCR analysis of a human-rodent somatic cell hybrid panel. Thus, like other proteins that are components of or control the structure of chromatin, Spt4p appears to be conserved from S. cerevisiae to mammals.
Spt4p是酿酒酵母中的一种非组蛋白，据信它对于正常的染色质结构和转录是必需的。在这项工作中，我们描述了一个人类基因SUPT4H的分离和分析，该基因编码一种预测的蛋白质，与Spt4p有42%的同一性。当在酿酒酵母中表达时，SUPT4H弥补了所有spt4突变体表型。在人类细胞中，SUPT4H编码一种在所有测试组织中都有表达的核蛋白。此外，杂交分析表明小鼠中也存在一个与SUPT4H相关的基因。通过对人-啮齿动物体细胞杂交板进行PCR分析，SUPT4H被定位到人类17号染色体上。因此，与其他作为染色质组成部分或控制染色质结构的蛋白质一样，Spt4p似乎从酿酒酵母到哺乳动物都是保守的。
