von Troil-Lindén B, Torkko H, Alaluusua S, Jousimies-Somer H, Asikainen S
Department of Periodontology, University of Helsinki, Finland.
J Dent Res. 1995 Nov;74(11):1789-95. doi: 10.1177/00220345950740111201.
The primary ecological niche for suspected periodontal pathogens seems to be the subgingival area, even though periodontal pathogens are also frequently recovered from saliva. The interrelationship of different periodontal conditions and the salivary levels of suspected periodontal pathogens is not known. In the present study, salivary levels of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Campylobacter rectus, and Peptostreptococcus micros were determined by bacterial culture and related to clinical periodontal status in 40 subjects with either advanced, moderate, or initial/no periodontitis. Culture-positive subjects harbored the 5 bacterial species in mean numbers ranging from 2 x 10(5) to 6 x 10(7) colony-forming units (CFU)/mL saliva. A. actinomycetemcomitans was found in none and P. gingivalis in one of the subjects with initial periodontitis, whereas both species were found in 33% and 44%, respectively, of the subjects with moderate periodontitis and in 60% and 40%, respectively, of the subjects with advanced periodontitis. The mean numbers of CFU/mL of P. intermedia, C. rectus and P. micros were significantly higher in subjects with advanced periodontitis than in subjects with initial/no periodontitis. Ten patients with advanced periodontitis were treated mechanically and with adjunctive systemic metronidazole, and were re-examined 1 and 6 months after treatment. Periodontal treatment eradicated or significantly reduced the levels of salivary periodontal pathogens for half a year, whereas in untreated subjects, the levels and the detection frequencies generally remained fairly stable. In conclusion, the results showed that the salivary levels of periodontal pathogens reflect the periodontal status of the patient.
尽管牙周病原体也经常从唾液中检出，但其主要生态位似乎是龈下区域。不同牙周状况与可疑牙周病原体唾液水平之间的相互关系尚不清楚。在本研究中，通过细菌培养测定了40名患有重度、中度或初期/无牙周炎患者唾液中伴放线放线杆菌、牙龈卟啉单胞菌、中间普氏菌、直肠弯曲菌和微小消化链球菌的水平，并将其与临床牙周状况相关联。培养阳性的受试者唾液中这5种细菌的平均数量范围为2×10⁵至6×10⁷菌落形成单位（CFU）/mL。在初期牙周炎患者中，未发现伴放线放线杆菌，仅1名患者检出牙龈卟啉单胞菌；而在中度牙周炎患者中，这两种菌的检出率分别为33%和44%，在重度牙周炎患者中分别为60%和40%。中间普氏菌、直肠弯曲菌和微小消化链球菌的CFU/mL平均数量在重度牙周炎患者中显著高于初期/无牙周炎患者。10名重度牙周炎患者接受了机械治疗并辅助全身应用甲硝唑，治疗后1个月和6个月进行复查。牙周治疗使唾液中牙周病原体水平在半年内消除或显著降低，而未治疗的受试者中，这些水平和检出频率通常保持相当稳定。总之，结果表明牙周病原体的唾液水平反映了患者的牙周状况。
