Mickelsen P A, McCarthy L R, Propst M A
J Clin Microbiol. 1977 Mar;5(3):297-301. doi: 10.1128/jcm.5.3.297-301.1977.
A modified auxanographic carbohydrate assimilation procedure for the identification of medically important yeasts is described. This method employs a heavy inoculum of unstarved yeasts, autoclaved yeast assimilation medium, pour plates of shallow depth, and commercially available carbohydrate-impregnated disks. The accuracy of this procedure was established in a comparison with the Wickerham broth method.
本文描述了一种改良的用于鉴定医学上重要酵母菌的生长谱碳水化合物同化程序。该方法采用未饥饿酵母菌的大量接种物、经高压灭菌的酵母菌同化培养基、浅深度的倾注平板以及市售的含碳水化合物的圆片。通过与威克汉姆肉汤法比较,确定了该程序的准确性。