Skelly P J, Shoemaker C B
Department of Tropical Public Health, Harvard School of Public Health, Boston, MA 02115, USA.
Mem Inst Oswaldo Cruz. 1995 Mar-Apr;90(2):281-4. doi: 10.1590/s0074-02761995000200027.
During their complex life cycle schistosomes alternate between the use of stored glycogen and reliance on host glucose to provide for their energy needs. In addition, there is dramatic variation between the relative contribution of aerobic versus anaerobic glucose metabolism during development. We have cloned a set of representative cDNAs that encode proteins involved in glucose uptake, glycolysis, Kreb's cycle and oxidative phosphorylation. The different cDNAs were used as probes to examine the expression of glucose metabolism genes during the schistosome life cycle. Steady state mRNA levels from whole cercariae, isolated cercarial tails, schistosomula and adult worms were analysed on Northern blots and dot blots which were quantified using storage phosphor technology. These studies reveal: (1) Transcripts encoding glycogen metabolic enzymes are expressed to much higher levels in cercarial tails than whole cercariae or schistosomula while the opposite pattern is found for glucose transporters and hexokinase transcripts; (2) Schistosomula contain low levels of transcripts encoding respiratory enzymes but regain the capacity for aerobic glucose metabolism as they mature to adulthood; (3) Male and female adults contain similar levels of the different transcripts involved in glucose metabolism.
在其复杂的生命周期中，血吸虫在利用储存的糖原和依赖宿主葡萄糖来满足其能量需求之间交替。此外，在发育过程中，有氧与无氧葡萄糖代谢的相对贡献存在显著差异。我们克隆了一组代表性的cDNA，它们编码参与葡萄糖摄取、糖酵解、三羧酸循环和氧化磷酸化的蛋白质。不同的cDNA被用作探针，以检测血吸虫生命周期中葡萄糖代谢基因的表达。使用存储磷光技术对Northern印迹和斑点印迹上的整个尾蚴、分离的尾蚴尾部、童虫和成虫的稳态mRNA水平进行了分析。这些研究揭示：（1）编码糖原代谢酶的转录本在尾蚴尾部的表达水平远高于整个尾蚴或童虫，而葡萄糖转运蛋白和己糖激酶转录本的情况则相反；（2）童虫中编码呼吸酶的转录本水平较低，但随着它们发育成熟为成虫，恢复了有氧葡萄糖代谢的能力；（3）成年雌雄虫中参与葡萄糖代谢的不同转录本水平相似。
