Nirmala C, Puvanakrishnan R
Department of Biotechnology, Central Leather Research Institute, Madras, India.
Biochem Pharmacol. 1996 Jan 12;51(1):47-51. doi: 10.1016/0006-2952(95)02118-3.
The effect of curcumin on lysosomal hydrolases in serum and heart was studied by determining the activities of beta-glucuronidase, beta-N-acetylglucosaminidase, cathepsin B, cathepsin D, and acid phosphatase. Rats treated with isoproterenol (30 mg/100 g body weight) showed a significant increase in serum lysosomal hydrolase activities, which were found to decrease after curcumin treatment. Isoproterenol administration to rats resulted in decreased stability of the membranes, which was reflected by the lowered activity of cathepsin D in mitochondrial, lysosomal, and microsomal fractions. Curcumin treatment returned the activity levels almost to normal, showing that curcumin restored the normal function of the membrane. Histopathological studies of the infarcted rat heart also showed a decreased degree of necrosis after curcumin treatment.
通过测定β-葡萄糖醛酸酶、β-N-乙酰氨基葡萄糖苷酶、组织蛋白酶B、组织蛋白酶D和酸性磷酸酶的活性，研究了姜黄素对血清和心脏中溶酶体水解酶的影响。用异丙肾上腺素（30mg/100g体重）处理的大鼠血清溶酶体水解酶活性显著增加，而在姜黄素处理后发现该活性降低。给大鼠注射异丙肾上腺素导致膜稳定性降低，这通过线粒体、溶酶体和微粒体部分中组织蛋白酶D活性降低得以体现。姜黄素处理使活性水平几乎恢复正常，表明姜黄素恢复了膜的正常功能。对梗死大鼠心脏的组织病理学研究还表明，姜黄素处理后坏死程度降低。
