Rocha L, Marston A, Potterat O, Kaplan M A, Stoeckli-Evans H, Hostettmann K
Institut de Pharmacognosie et Phytochimie, Université de Lausanne, Lausanne-Dorigny, Switzerland.
Phytochemistry. 1995 Nov;40(5):1447-52. doi: 10.1016/0031-9422(95)00507-4.
Three known phloroglucinols (japonicine A, uliginosin A and isouliginosin B) and a new phloroglucinol (hyperbrasiol A) have been isolated from a petrol extract of the leaves and flowers of Hypericum brasiliense. Their structures were established by spectroscopic methods (UV, DCI-MS, 1H and 13CNMR, including SINEPT, HMBC, HSQC, DQFCOSY experiments). The substitution pattern of hyperbrasilol A was confirmed by X-ray crystallography. All four phloroglucinols were antibacterial against Bacillus subtilis in a TLC bioautographic assay. The flavonoids, kaempferol, luteolin, quercetin, quercitrin, isoquercitrin, hyperoside and guaijaverin, were isolated from a methanol extract of the same organs.
从巴西金丝桃的叶和花的石油醚提取物中分离出了三种已知的间苯三酚（日本獐牙菜素A、沼生水马齿素A和异沼生水马齿素B）和一种新的间苯三酚（金丝桃新酚A）。通过光谱方法（紫外光谱、直接化学电离质谱、氢核磁共振和碳-13核磁共振，包括选择极化转移增强、异核多键相关、异核单量子相关、双量子滤波相关谱实验）确定了它们的结构。通过X射线晶体学确定了金丝桃新酚A的取代模式。在薄层层析生物自显影试验中，所有四种间苯三酚对枯草芽孢杆菌均具有抗菌活性。从同一器官的甲醇提取物中分离出了黄酮类化合物山柰酚、木犀草素、槲皮素、槲皮苷、异槲皮苷、金丝桃苷和桂樱苷。
