利用简单序列长度多态性对大鼠近交系进行遗传特征分析。

Use of simple sequence length polymorphisms for genetic characterization of rat inbred strains.

作者信息

Otsen M, Den Bieman M, Winer E S, Jacob H J, Szpirer J, Szpirer C, Bender K, Van Zutphen L F

机构信息

Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.

出版信息

Mamm Genome. 1995 Sep;6(9):595-601. doi: 10.1007/BF00352364.

DOI:10.1007/BF00352364

PMID:8535065

Abstract

Genetic monitoring is an essential component of colony management and for the rat has been accomplished primarily by using immunological and biochemical markers. Here, we report that simple sequence length polymorphisms (SSLPs) are a faster and more economical way of monitoring inbred strains of rats. We characterized 61 inbred strains of rats, using primer pairs for 37 SSLPs. Each of these loci appeared to be highly polymorphic, with the number of alleles per locus ranging between 3 and 14 and, as a result, all the 61 inbred strains tested in this study could be provided with a unique strain profile. These strain profiles are also used for estimating the degree of similarity between strains. This information may provide the rationale in selecting strains for genetic crosses or for other specific purposes.

摘要

基因监测是群体管理的重要组成部分，对于大鼠而言，主要通过使用免疫和生化标记来完成。在此，我们报告简单序列长度多态性（SSLPs）是监测近交系大鼠的一种更快且更经济的方法。我们使用针对37个SSLPs的引物对，对61个近交系大鼠进行了特征分析。这些位点中的每一个似乎都具有高度多态性，每个位点的等位基因数量在3至14个之间，因此，本研究中测试的所有61个近交系都可以获得独特的品系图谱。这些品系图谱还用于估计品系之间的相似程度。该信息可为选择用于遗传杂交或其他特定目的的品系提供理论依据。