使用质子核磁共振波谱法分析胎儿和新生儿尿液。
Analysis of fetal and neonatal urine using proton nuclear magnetic resonance spectroscopy.
作者信息
Foxall P J, Bewley S, Neild G H, Rodeck C H, Nicholson J K
机构信息
Department of Chemistry, Birkbeck College, University of London.
出版信息
Arch Dis Child Fetal Neonatal Ed. 1995 Nov;73(3):F153-7. doi: 10.1136/fn.73.3.f153.
AIM
To use high field proton nuclear magnetic resonance spectroscopy (1H NMR) to characterise the low molecular weight metabolite composition of neonatal and fetal urine in relation to gestational age and perinatal outcome.
METHODS
The first urine passed by two neonatal groups, six full term and five preterm infants with normal renal function, was analysed by 1H NMR and compared with fetal urine from 14 cases with obstructive uropathy.
RESULTS
The mean ratios of taurine, myo-inositol, and trimethylamine-N-oxide (TMAO) to creatinine were 4.3, 10.1, and 14.1 times higher, respectively, in the preterm group when compared with those of the full term group. Fetal obstructive uropathy was characterised by glycosuria, amino and organic aciduria, regardless of gestational age (13-30 weeks).
CONCLUSIONS
Samples of the first urine passed--that is, urine produced in fetal life--by normal preterm infants are useful controls for cases of obstructive uropathy detected in the third trimester. 1H NMR will become a clinically useful tool for monitoring renal development and abnormalities in utero.
目的
运用高场质子核磁共振波谱法（1H NMR）来表征新生儿及胎儿尿液中的低分子量代谢物组成，及其与胎龄和围产期结局的关系。
方法
对两组新生儿（六名足月且肾功能正常的婴儿和五名早产且肾功能正常的婴儿）排出的首次尿液进行1H NMR分析，并与14例患有梗阻性肾病的胎儿尿液进行比较。
结果
与足月组相比，早产组中牛磺酸、肌醇和氧化三甲胺（TMAO）与肌酐的平均比值分别高出4.3倍、10.1倍和14.1倍。胎儿梗阻性肾病的特征为糖尿、氨基酸尿和有机酸尿，与胎龄（13 - 30周）无关。
结论
正常早产婴儿排出的首次尿液样本，即胎儿期产生的尿液，是孕晚期检测到的梗阻性肾病病例的有用对照。1H NMR将成为监测子宫内肾脏发育和异常情况的临床有用工具。
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