新型小鼠微卫星：引物序列及染色体定位

Novel mouse microsatellites: primer sequences and chromosomal location.

作者信息

Suda T, Oyanagi M, Wakana S, Takahashi Y, Kanada H, Yonekawa H, Miyashita N, Shiroishi T, Moriwaki K, Kominami R

机构信息

First Department of Biochemistry, Niigata University School of Medicine, Japan.

出版信息

DNA Res. 1994;1(4):169-74. doi: 10.1093/dnares/1.4.169.

DOI:10.1093/dnares/1.4.169

PMID:8535974

Abstract

Sixty-nine sequences containing microsatellites were determined by analysis of clones from a pUC118 library of total genomic mouse DNA. These sequences were examined for size variation using polymerase chain reaction and gel electrophoresis. Fifty-one of them showed allelic variations between C57BL/6 and MSM, the two strains used for genetic mapping. Hence, their chromosomal location was determined using a panel consisting of 131 backcross mice that had been typed with 85 anchor loci. The microsatellites were distributed to most chromosomes except for chromosomes 16 and 19. These novel markers with defined locations are useful in linkage and genome mapping studies.

摘要

通过对来自小鼠全基因组DNA的pUC118文库的克隆进行分析，确定了69个含有微卫星的序列。使用聚合酶链反应和凝胶电泳检测这些序列的大小变异。其中51个在用于基因定位的C57BL/6和MSM这两个品系之间表现出等位基因变异。因此，利用由131只已用85个锚定基因座分型的回交小鼠组成的群体确定了它们的染色体位置。微卫星分布于除16号和19号染色体之外的大多数染色体上。这些具有明确位置的新型标记物在连锁和基因组作图研究中很有用。