清酒乳杆菌0-1产生的胞外多糖结构的测定
Determination of the structure of the exopolysaccharide produced by Lactobacillus sake 0-1.
作者信息
Robijn G W, van den Berg D J, Haas H, Kamerling J P, Vliegenthart J F
机构信息
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
出版信息
Carbohydr Res. 1995 Oct 16;276(1):117-36. doi: 10.1016/0008-6215(95)00172-p.
The exopolysaccharide produced by Lactobacillus sake 0-1 in a semi-defined medium was found to have an average molecular mass of 6 x 10(6) Da and a composition of D-glucose, L-rhamnose, and sn-glycerol 3-phosphate (3:2:1). The polysaccharide is partially O-acetylated. By means of partial acid hydrolysis, O-deacetylation, deglycerophosphorylation, methylation analysis, and 1D/2D NMR (1H, 13C, and 31P) studies the polysaccharide was shown to be composed of repeating units with the following structure: [formula: see text]
在半限定培养基中，清酒乳杆菌0-1产生的胞外多糖的平均分子量为6×10⁶Da，其组成成分为D-葡萄糖、L-鼠李糖和sn-甘油3-磷酸（3:2:1）。该多糖部分被O-乙酰化。通过部分酸水解、O-脱乙酰化、去甘油磷酸化、甲基化分析以及一维/二维核磁共振（¹H、¹³C和³¹P）研究表明，该多糖由具有以下结构的重复单元组成：[分子式：见原文]
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