Stingele F, Lemoine J, Neeser J R
Nestlé Research Center, Lausanne, Switzerland.
Carbohydr Res. 1997 Aug 7;302(3-4):197-202. doi: 10.1016/s0008-6215(97)00119-5.
Lactobacillus helveticus Lh59 produces a high-molecular-mass exopolysaccharide (> or = 2 x 10(6) Da) when cultured in skimmed milk. Compositional analysis, methylation analysis and NMR experiments (1H and 13C) recorded from the native polysaccharide as well as from oligosaccharides released by partial acid hydrolysis, allowed the complete structural determination of this polysaccharide, which consists of the following hexasaccharide repeating unit: [symbol: see text] This structure is identical to the one of an EPS produced by L. helveticus TN-4, which was claimed to be a spontaneous mutant of strain TY1-2.
瑞士乳杆菌Lh59在脱脂乳中培养时会产生一种高分子量胞外多糖(≥2×10⁶ Da)。对天然多糖以及部分酸水解释放出的寡糖进行的成分分析、甲基化分析和核磁共振实验(¹H和¹³C),使得该多糖的完整结构得以确定,其由以下六糖重复单元组成:[符号:见原文] 此结构与瑞士乳杆菌TN-4产生的一种胞外多糖的结构相同,TN-4被认为是菌株TY1-2的自发突变体。