Sakamoto J, Nakaji S, Sugawara K, Iwane S, Munakata A
Department of Hygiene, Hirosaki University School of Medicine, Japan.
Gastroenterology. 1996 Jan;110(1):116-20. doi: 10.1053/gast.1996.v110.pm8536846.
BACKGROUND & AIMS: Epidemiological and experimental studies have shown that dietary fiber may prevent colon cancer. Resistant starch, like dietary fiber, is not subject to digestion in the small intestine. However, it is unknown whether resistant starch inhibits colonic carcinogenesis. In vitro studies have shown that butyrate slows the growth of cultured colon cancer cells. The effect of resistant starch diet on 1,2-dimethylhydrazine-induced colonic carcinogenesis in rats was evaluated, and the colonic butyrate concentration was measured.
Sprague-Dawley rats were randomly divided into five groups that were fed diets containing no fiber, 3% cellulose, 10% cellulose, 3% resistant starch, or 10% resistant starch. Colonic carcinogenesis and butyrate concentration of colonic contents and feces in each diet group were compared.
Total cancer volume per rat in the 10% cellulose group was significantly lower than that in the basal group (109 +/- 54 mm3 and 247 +/- 83 mm3; P < 0.05), but the other groups showed no significant differences. The butyrate concentration in colonic content and in feces were higher in the resistant starch groups than in the cellulose groups.
The resistant starch diet increased butyrate concentration but did not inhibit colonic carcinogenesis. It remains doubtful whether butyrate inhibits the proliferation of colon cancer cells.
流行病学和实验研究表明膳食纤维可能预防结肠癌。抗性淀粉与膳食纤维一样，在小肠中不被消化。然而，抗性淀粉是否能抑制结肠癌发生尚不清楚。体外研究表明丁酸盐可减缓培养的结肠癌细胞的生长。本研究评估了抗性淀粉饮食对1,2 - 二甲基肼诱导的大鼠结肠癌发生的影响，并测量了结肠丁酸盐浓度。
将斯普拉格 - 道利大鼠随机分为五组，分别给予不含纤维、含3%纤维素、10%纤维素、3%抗性淀粉或10%抗性淀粉的饮食。比较各饮食组的结肠癌发生情况以及结肠内容物和粪便中的丁酸盐浓度。
10%纤维素组每只大鼠的肿瘤总体积显著低于基础组（分别为109±54立方毫米和247±83立方毫米；P<0.05），但其他组无显著差异。抗性淀粉组结肠内容物和粪便中的丁酸盐浓度高于纤维素组。
抗性淀粉饮食增加了丁酸盐浓度，但未抑制结肠癌发生。丁酸盐是否能抑制结肠癌细胞的增殖仍存疑问。
