Becker J, Heun M
Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
Genome. 1995 Oct;38(5):991-8. doi: 10.1139/g95-130.
The broad use of microsatellites as a tool for constructing linkage maps in plants has been limited by the need for sequence data to detect the underlying simple sequence repeats. Therefore, random amplified microsatellite polymorphisms (RAMPs) were studied as an alternative approach for barely mapping. Labelled (GA)n simple sequence repeat primers were combined with RAPD primers of different length and sequence to generate RAMPs. To get additional polymorphisms (called dRAMPs), the obtained products were also analysed after digestion with MseI. There were 0-11 polymorphisms found per primer combination. Sixty RAMPs/dRAMPs identifying 40 new loci were mapped onto a barley RFLP map. The new DNA markers are found on all chromosomes and they increased the length of the barely map by 174 cM to a total of 1270 cM. Interestingly, the RAMPs/dRAMPs caused stretching effects in genome areas where stretching was also observed for AFLPs.
微卫星作为构建植物连锁图谱的工具，其广泛应用受到检测潜在简单序列重复所需序列数据的限制。因此，研究了随机扩增多态性微卫星（RAMPs）作为大麦作图的替代方法。将标记的（GA）n简单序列重复引物与不同长度和序列的RAPD引物组合以产生RAMPs。为了获得额外的多态性（称为dRAMPs），用MseI消化后也对所得产物进行分析。每个引物组合发现0至11个多态性。将鉴定出40个新位点的60个RAMPs/dRAMPs定位到大麦RFLP图谱上。新的DNA标记存在于所有染色体上，它们使大麦图谱的长度增加了174厘摩，达到总共1270厘摩。有趣的是，RAMPs/dRAMPs在AFLP也观察到拉伸的基因组区域中引起拉伸效应。
