Li J Z, Sjakste T G, Röder M S, Ganal M W
Institut für Pflanzengenetik und Kulturpflanzenforschung, Correnstrasse 3, 06466 Gatersleben, Germany.
Theor Appl Genet. 2003 Oct;107(6):1021-7. doi: 10.1007/s00122-003-1345-6. Epub 2003 Jul 16.
To enhance the marker density of existing genetic maps of barley ( Hordeum vulgare L.), a new set of microsatellite markers containing dinucleotide motifs was developed from genomic clones. Out of 254 primer pairs tested, a total of 167 primer pairs were classifed as functional in a panel of six barley cultivars and three H. spontaneum accessions, and of those, 127 primer pairs resulting in 133 loci were either mapped or located onto chromosomes. The polymorphism information content (PIC) ranged from 0.05 to 0.94 with an average of 0.60. The number of alleles per locus varied from 1 to 9. On average, 3.9 alleles per primer pair were observed. The RFLP frameworks of two previously published linkage maps were used to locate a total of 115 new microsatellite loci on at least one mapping population. The chromosomal assignment of 48 mapped loci was corroborated on a set of wheat-barley chromosome addition lines; 18 additional loci which were not polymorphic in the mapping populations were assigned to chromosomes by this method. The microsatellites were located on all seven linkage groups with four significant clusters in the centromeric regions of 2H, 3H, 6H and 7H. These newly developed microsatellites improve the density of existing barley microsatellite maps and can be used in genetic studies and breeding research.
为提高大麦（Hordeum vulgare L.）现有遗传图谱的标记密度，从基因组克隆中开发了一组新的含二核苷酸基序的微卫星标记。在测试的254对引物中，共有167对引物在一组六个大麦品种和三个野生大麦材料中被分类为功能性引物，其中127对引物产生了133个位点，这些位点已被定位到染色体上或确定其在染色体上的位置。多态性信息含量（PIC）范围为0.05至0.94，平均为0.60。每个位点的等位基因数从1到9不等。平均每对引物观察到3.9个等位基因。利用两个先前发表的连锁图谱的RFLP框架，在至少一个作图群体中定位了总共115个新的微卫星位点。在一组小麦-大麦染色体附加系上证实了48个定位位点的染色体归属；通过这种方法将另外18个在作图群体中无多态性的位点定位到染色体上。这些微卫星位于所有七个连锁群上，在2H、3H、6H和7H的着丝粒区域有四个明显的簇。这些新开发的微卫星提高了现有大麦微卫星图谱的密度，可用于遗传研究和育种研究。
