Turner A, Gough K R, Jephcott A E
PHLS Gonococcus Reference Unit, Public Health Laboratory, Bristol Royal Infirmary.
J Clin Pathol. 1995 Oct;48(10):919-23. doi: 10.1136/jcp.48.10.919.
To establish the current sensitivity of two commercial kits for culture confirmation of Neisseria gonorrhoeae strains circulating in the UK.
A total of 544 strains were studied (199 gonococci from male patients attending hospitals in the county of Avon, 204 unselected N gonorrhoeae isolates from male patients in Leeds, 20 strains referred to the Gonococcus Reference Unit because of difficulties with the Syva fluorescent antibody test (FAT), and 121 strains collected over a four year period which had not reacted with serotyping antibodies). Strains were tested by sugar utilisation in cysteine trypticase base agar (CTA test), the Phadebact Monoclonal GC Test and Syva MicroTrak Neisseria gonorrhoeae Culture Confirmation Test. The auxotype and serovar of each strain were also determined.
The sugar utilisation test confirmed the identity of 99% (197/199) of gonococci from Avon and 97% (198/204) of those from Leeds. The Syva FAT confirmed 76% (151/199) of isolates from Avon and 84% (171/204) of those from Leeds. The Phadebact test confirmed all but one isolate from the 403 strains from both cities. Half of the 20 referred FAT negative isolates also give a negative result in the Syva FAT; however, only 10% of the remainder gave a strong reaction in our laboratory. All serotyping antibody negative strains were negative in the FAT, although all these and all of the 20 strains that give a negative result in the FAT gave positive reactions in the other culture confirmation tests. Typing tests revealed a greater diversity amongst the FAT negative strains from Leeds than those from Avon.
Considerable differences in the sensitivity of the MicroTrak but not with the Phadebact or CTA tests were found for the identification of isolates from two geographically distinct areas of the UK. Our results suggest that the Syva FAT would not be suitable, if used alone, for culture confirmation in Avon or Leeds.
确定两种商用试剂盒对英国流行的淋病奈瑟菌菌株进行培养鉴定的当前敏感性。
共研究了544株菌株（199株来自埃文郡医院男性患者的淋球菌，204株从利兹市男性患者中随机选取的淋病奈瑟菌分离株，20株因Syva荧光抗体试验（FAT）困难而送交淋球菌参考单位的菌株，以及121株在四年期间收集的与血清分型抗体无反应的菌株）。通过在半胱氨酸胰蛋白酶基础琼脂中进行糖利用试验（CTA试验）、Phadebact单克隆GC试验和Syva MicroTrak淋病奈瑟菌培养鉴定试验对菌株进行检测。还确定了每个菌株的营养型和血清型。
糖利用试验确认了来自埃文郡99%（197/199）的淋球菌和来自利兹市97%（198/204）的淋球菌的身份。Syva FAT确认了来自埃文郡76%（151/199）的分离株和来自利兹市84%（171/204）的分离株。Phadebact试验确认了来自两个城市的403株菌株中除一株外的所有分离株。20株送交的FAT阴性分离株中有一半在Syva FAT中也呈阴性结果；然而，其余分离株中只有10%在我们实验室中呈强反应。所有血清分型抗体阴性菌株在FAT中均为阴性，尽管所有这些菌株以及在FAT中呈阴性结果的所有20株菌株在其他培养鉴定试验中均呈阳性反应。分型试验显示，来自利兹市的FAT阴性菌株比来自埃文郡的菌株具有更大的多样性。
在鉴定来自英国两个地理区域的分离株时，发现MicroTrak的敏感性存在显著差异，但Phadebact或CTA试验不存在这种差异。我们的结果表明，如果单独使用，Syva FAT不适用于埃文郡或利兹市的培养鉴定。
