Fukuzawa K, Horikoshi T
Department of Dermatology, Sapporo Medical College, Japan.
Br J Dermatol. 1992 Apr;126(4):324-30. doi: 10.1111/j.1365-2133.1992.tb00673.x.
The effect of HuIFN-beta on the invasive potential of melanoma cells was studied using an in-vitro model system with Transwell chambers equipped with matrigel-coated polycarbonate filters. When (10(2), 10(3) and 10(4) IU/ml) for 3 days and then grown in medium without HuIFN-beta for another 7 days. On day 7, the proliferation of melanoma cells was inhibited by 77 and 87%, respectively, when cells were treated with 10(2) and 10(4) IU/ml of HuIFN-beta. This antiproliferative effect was dose-dependent and more pronounced on day 11. The effect of HuIFN-beta on the invasive potential of melanoma cells was studied using an in-vitro model system with Transwell chambers equipped with Matrigel-coated polycarbonate filters. When cells were treated with HuIFN-beta (10(2), 10(3) and 10(4) IU/ml) for 24 h, the amount of tritiated thymidine incorporated into cells was increased, indicating that cell growth was not inhibited. However, the number of cells that invaded to the filter decreased significantly by 15-40%. HuIFN-beta did not have an inhibitory effect on the haptotactic migration of melanoma cells. These data indicate that the antiproliferative effect of HuIFN-beta occurs after 24 h and that the direct anti-invasive effect is independent of any effect on proliferation.
使用配备有基质胶包被的聚碳酸酯滤膜的Transwell小室体外模型系统，研究了人干扰素β（HuIFN-β）对黑色素瘤细胞侵袭潜能的影响。当用10²、10³和10⁴国际单位/毫升的HuIFN-β处理细胞3天，然后在不含HuIFN-β的培养基中再培养7天时。在第7天，当用10²和10⁴国际单位/毫升的HuIFN-β处理细胞时，黑色素瘤细胞的增殖分别被抑制了77%和87%。这种抗增殖作用呈剂量依赖性，且在第11天更为明显。使用配备有基质胶包被的聚碳酸酯滤膜的Transwell小室体外模型系统，研究了HuIFN-β对黑色素瘤细胞侵袭潜能的影响。当用HuIFN-β（10²、10³和10⁴国际单位/毫升）处理细胞24小时时，掺入细胞中的氚标记胸腺嘧啶核苷的量增加，表明细胞生长未受抑制。然而，侵袭到滤膜上的细胞数量显著减少了15%至40%。HuIFN-β对黑色素瘤细胞的趋触性迁移没有抑制作用。这些数据表明，HuIFN-β的抗增殖作用在24小时后出现，且直接的抗侵袭作用独立于对增殖的任何影响。
