Morimoto Y, Saga K
Department of Dermatology, Sapporo Medical University School of Medicine, Japan.
J Histochem Cytochem. 1995 Dec;43(12):1217-21. doi: 10.1177/43.12.8537637.
Morphological observations of sweat glands showed degenerated debris of secretory cells in the secretory lumen in both apocrine and eccrine sweat glands. This suggested that dead secretory cells of human eccrine and apocrine sweat glands were released into the lumen and replaced by other cells. However, we did not know which type of cells replaced lost secretory cells. Therefore, we studied the proliferating cells in human eccrine and apocrine sweat glands by labeling S-phase cells in vitro with 5-bromo-2'-deoxyuridine (BrdUrd) and by immunostaining proliferation-associated proliferating cell nuclear antigen (PCNA) with anti-PCNA monoclonal antibody. BrdUrd and anti-PCNA antibody labeled a few secretory cells in eccrine and apocrine sweat glands, but neither method labeled myoepithelial cells. Luminal and peripheral cells of the eccrine and apocrine coiled duct were labeled with both BrdUrd and PCNA. However, we could not find any highly proliferative germinative cells in coiled ducts. Our results suggest that lost secretory cells could be replaced by proliferation of secretory cells themselves rather than by proliferation of myoepithelial cells or duct cells.
汗腺的形态学观察显示，顶泌汗腺和小汗腺的分泌管腔中均有分泌细胞的退化碎片。这表明人类小汗腺和顶泌汗腺死亡的分泌细胞被释放到管腔中，并被其他细胞取代。然而，我们不知道是哪种类型的细胞取代了丢失的分泌细胞。因此，我们通过用5-溴-2'-脱氧尿苷（BrdUrd）体外标记S期细胞，并使用抗增殖细胞核抗原（PCNA）单克隆抗体对增殖相关的增殖细胞核抗原进行免疫染色，来研究人类小汗腺和顶泌汗腺中的增殖细胞。BrdUrd和抗PCNA抗体标记了小汗腺和顶泌汗腺中的一些分泌细胞，但两种方法均未标记肌上皮细胞。小汗腺和顶泌汗腺蟠曲导管的管腔细胞和外周细胞均被BrdUrd和PCNA标记。然而，我们在蟠曲导管中未发现任何高度增殖的生发细胞。我们的结果表明，丢失的分泌细胞可能由分泌细胞自身的增殖所取代，而非由肌上皮细胞或导管细胞的增殖所取代。
