Tsai C C, Ho Y P, Chen C C
Department of Periodontology, School of Dentistry, Kaohsiung Medical College, Taiwan, ROC.
J Periodontol. 1995 Oct;66(10):852-9. doi: 10.1902/jop.1995.66.10.852.
Recent in vitro findings indicate that cytokines represent an important pathway of connective tissue destruction in human periodontitis. The biological effects of interleukin-1 beta (IL-1 beta) and interleukin-8 (IL-8) are relevant in this regard, and the objective of this study was to compare the levels of these molecules in gingival crevicular fluids (GCF) from patients with adult periodontitis (experimental group) and from individuals with clinically healthy gingiva (control group). GCF was collected for 30 seconds using a periopaper strip and the volume of the sample determined. Following elution of the fluid, assays for IL-1 beta and IL-8 were carried out by ELISA. The concentrations (ng/ml) of cytokines were calculated in the original volume of GCF on each strip. The total amounts (pg/site) of cytokines were expressed as the concentrations multiplied by volumes of GCF: The total amounts of IL-1 beta and IL-8 of the experimental group were significantly higher than the control group. The total amounts of both cytokines were markedly reduced following phase 1 periodontal treatment. The clinical parameters were positively related to the total amounts of IL-1 beta and IL-8. IL-1 beta concentrations and total amounts were also positively related to IL-8 suggesting that the GCF IL-8 levels are influenced by local IL-1 beta activities. These data indicate that the total amounts of IL-1 beta and IL-8 exhibited dynamic changes upon severity of periodontal disease. The levels of IL-1 beta and IL-8 in GCF are valuable in detecting the inflammation of periodontal tissue.
最近的体外研究结果表明，细胞因子是人类牙周炎中结缔组织破坏的重要途径。白细胞介素-1β（IL-1β）和白细胞介素-8（IL-8）的生物学效应在这方面具有相关性，本研究的目的是比较成人牙周炎患者（实验组）和临床健康牙龈个体（对照组）龈沟液（GCF）中这些分子的水平。使用牙周试纸条收集GCF 30秒，并测定样品体积。洗脱液体后，通过酶联免疫吸附测定法（ELISA）对IL-1β和IL-8进行检测。计算每条试纸条上GCF原始体积中细胞因子的浓度（ng/ml）。细胞因子的总量（pg/部位）表示为浓度乘以GCF体积：实验组中IL-1β和IL-8的总量显著高于对照组。在牙周治疗第一阶段后，两种细胞因子的总量均显著降低。临床参数与IL-1β和IL-8的总量呈正相关。IL-1β浓度和总量也与IL-8呈正相关，这表明GCF中IL-8水平受局部IL-1β活性影响。这些数据表明，IL-1β和IL-8的总量随牙周疾病严重程度呈现动态变化。GCF中IL-1β和IL-8水平对于检测牙周组织炎症具有重要价值。
