Vegesna V, McBride W H, Taylor J M, Withers H R
Department of Radiation Oncology, University of California at Los Angeles 90024-1714, USA.
J Surg Res. 1995 Dec;59(6):699-704. doi: 10.1006/jsre.1995.1226.
The ability of exogenous interleukin-1 beta (IL-1 beta) or transforming growth factor-beta (TGF-beta) to reverse radiation-induced defective wound healing was investigated. Mice were irradiated with 8.5 or 11 Gy to total body (TB), 12 or 16 Gy to hemibody (HB), or 20 or 26 Gy to skin alone immediately prior to surgical wounding in order to determine the effect of hematopoietic system depletion on cytokine action. A single dose of IL-1 beta or TGF-beta or vehicle control was applied to each wound. All radiation doses and types resulted in a deficit in wound healing when measured on Days 11 and 14. IL-1 beta enhanced wound tensile strength (WTS) in TB-irradiated mice, while TGF-beta enhanced WTS in HB-irradiated mice. Neither cytokine was effective at enhancing WTS in unirradiated or skin-only irradiated animals. In addition IL-1 beta and TGF-beta showed distinct differences in their effects on the kinetics of healing with time after wounding. The effects of TGF-beta appeared to be transient with compromise of the gain in WTS. The differences in the effects of these two cytokines in affecting wound healing reflect their involvement in different cellular events in the wound healing process.
研究了外源性白细胞介素-1β（IL-1β）或转化生长因子-β（TGF-β）逆转辐射诱导的伤口愈合缺陷的能力。在手术创伤前，对小鼠进行全身（TB）8.5或11 Gy、半身（HB）12或16 Gy或仅皮肤20或26 Gy的照射，以确定造血系统耗竭对细胞因子作用的影响。对每个伤口给予单剂量的IL-1β或TGF-β或载体对照。在第11天和第14天测量时，所有辐射剂量和类型均导致伤口愈合缺陷。IL-1β增强了TB照射小鼠的伤口抗张强度（WTS），而TGF-β增强了HB照射小鼠的WTS。两种细胞因子在未照射或仅皮肤照射的动物中均不能有效增强WTS。此外，IL-1β和TGF-β在伤口愈合动力学方面的作用随时间显示出明显差异。TGF-β的作用似乎是短暂的，导致WTS增加受损。这两种细胞因子在影响伤口愈合方面的作用差异反映了它们参与了伤口愈合过程中不同的细胞事件。
