培养微量滴定法：一种用于定量检测感染小鼠组织中婴儿利什曼原虫的灵敏方法。
Culture microtitration: a sensitive method for quantifying Leishmania infantum in tissues of infected mice.
作者信息
Buffet P A, Sulahian A, Garin Y J, Nassar N, Derouin F
机构信息
Laboratoire de Parasitologie-Mycologie, Faculté de Médecine Lariboisière Saint-Louis, Paris, France.
出版信息
Antimicrob Agents Chemother. 1995 Sep;39(9):2167-8. doi: 10.1128/AAC.39.9.2167.
Abstract
We developed a microtitration method to determine the parasite burdens in homogenized organs of mice infected with Leishmania infantum. This method proved more sensitive than direct enumeration of amastigotes in stained organs, was appropriate for describing the kinetics of infection, and can be considered for physiopathological or pharmaceutical experimental studies.
摘要
我们开发了一种微量滴定法来测定感染婴儿利什曼原虫的小鼠匀浆器官中的寄生虫负荷。该方法比直接计数染色器官中的无鞭毛体更敏感，适用于描述感染动力学，可用于生理病理学或药物实验研究。
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本文引用的文献
1
Leishmaniasis and AIDS co-infection: the Spanish example.利什曼病与艾滋病合并感染：以西班牙为例。
Parasitol Today. 1994 Apr;10(4):160-3. doi: 10.1016/0169-4758(94)90270-4.
2
Advantages of measuring changes in the number of viable parasites in murine models of experimental cutaneous leishmaniasis.在实验性皮肤利什曼病小鼠模型中测量活寄生虫数量变化的优势。
Infect Immun. 1983 Mar;39(3):1087-94. doi: 10.1128/iai.39.3.1087-1094.1983.
3
A limiting dilution assay for quantifying Leishmania major in tissues of infected mice.一种用于定量感染小鼠组织中硕大利什曼原虫的有限稀释分析方法。
Parasite Immunol. 1985 Sep;7(5):545-55. doi: 10.1111/j.1365-3024.1985.tb00098.x.
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