Heinze P A, Thrupp L D, Anselmo C R
Am J Clin Pathol. 1979 Feb;71(2):177-83. doi: 10.1093/ajcp/71.2.177.
This study evaluates a new direct rapid system for urine cultures, including detection and quantitation of positive specimens by Gram stain, direct identification by 4--6-hour incubation of sediment with reagent strips, and antibiotic susceptibility testing by direct (3--4-hour) disk-elution methods. Of 987 routine urine specimens, 121 had significant (less than or equal to 10(5) colony-forming units/ml) gram-negative bacilluria, of which 89% were detected by the Gram stain. Direct rapid identification was correct in 94%. Results of direct disk-elution antimicrobial tests showed overall agreement with results of standard disk diffusion of 93% of tests, and major discrepancies in 4%. For urine specimens with gram-negative bacilluria, this system permitted detection, quantitation, identification, and antimicrobial susceptibility testing in four to six hours with reasonable, though not complete, accuracy.
本研究评估了一种用于尿培养的新型直接快速系统，包括通过革兰氏染色对阳性标本进行检测和定量、用试剂条对沉淀物进行4 - 6小时孵育以直接鉴定以及通过直接（3 - 4小时）纸片洗脱法进行抗生素敏感性测试。在987份常规尿标本中，121份有显著（小于或等于10⁵菌落形成单位/毫升）革兰氏阴性杆菌尿，其中89%通过革兰氏染色检测到。直接快速鉴定的正确率为94%。直接纸片洗脱抗菌试验结果与标准纸片扩散试验结果总体一致率为93%，主要差异率为4%。对于有革兰氏阴性杆菌尿的尿标本，该系统能在4至6小时内进行检测、定量、鉴定和抗菌敏感性测试，准确性合理但不完全准确。
