Murray P R, Niles A C
J Clin Microbiol. 1981 Jan;13(1):85-88. doi: 10.1128/jcm.13.1.85-88.1981.
We assessed two procedures for the initial processing of urine specimens: (i) manual screening of urine samples by inoculation into tryptic soy broth and incubation for 3 to 6 h, and (ii) examination of agar plates inoculated with urine and incubated for 4 to 6 h. Of the urine samples with greater than 10(5) colony-forming units per ml, 73.6 and 90.1% were detected with the broth cultures after 3 and 6 h of incubation, respectively. The organisms not detected at 6 h were Corynebacterium spp., Lactobacillus spp., and one of six isolates of yeasts. When inoculated plates were examined for growth, 53.3 and 80.0% of the specimens with greater than 10(5) colony-forming units per ml were detected after 4 and 6 h of incubation, respectively. Of the cultures with significant growth of Enterobacteriaceae or Pseudomonas spp., 95% were detected on the plates after 6 h of incubation.
（i）通过接种到胰蛋白胨大豆肉汤中并孵育3至6小时对尿液样本进行手工筛查，以及（ii）检查接种尿液并孵育4至6小时的琼脂平板。每毫升菌落形成单位大于10⁵的尿液样本中，分别在孵育3小时和6小时后，用肉汤培养法检测到的比例为73.6%和90.1%。在6小时未检测到的微生物为棒状杆菌属、乳杆菌属，以及6株酵母分离株中的1株。当检查接种平板的生长情况时，每毫升菌落形成单位大于10⁵的标本中，分别在孵育4小时和6小时后检测到的比例为53.3%和80.0%。在肠杆菌科或假单胞菌属有显著生长的培养物中，95%在孵育6小时后在平板上被检测到。
