Winkler T, Sharma H S, Stålberg E, Olsson Y, Dey P K
Department of Clinical Neurophysiology, University Hospital, Uppsala, Sweden.
Neuroscience. 1995 Oct;68(4):1097-104. doi: 10.1016/0306-4522(95)00194-n.
The possibility that elevation of serotonin in the circulation, which is found in various pathological states, influences the spontaneous cerebral cortical activity was examined in a rat model. The electroencephalogram was recorded using bipolar epidural electrodes placed over the frontal and parietal cerebral cortex. Intravenous infusion of serotonin (10 micrograms/kg per min for 10 min) decreased the electroencephalogram amplitude in both frontal and parietal recordings within 4 min of infusion. This decrease in amplitude was reversible, Pretreatment with cyproheptadine (a potent serotonin2 receptor antagonist) prevented the serotonin-induced decrease of the electroencephalogram amplitude. The blood-brain barrier permeability to Evans Blue and [131I]sodium was increased in frontal and parietal cortex. This increase in blood-brain barrier permeability was absent in animals pretreated with cyproheptadine. These results provide direct evidence that an elevated level of serotonin in blood has the capacity to influence spontaneous cortical electrical activity. This effect of serotonin on electroencephalogram appears to be due to its ability to enter into the brain parenchyma by inducing a short-term breakdown of the blood-brain barrier, probably via serotonin2 receptors.
在大鼠模型中研究了各种病理状态下循环中血清素升高影响大脑皮质自发活动的可能性。使用置于额叶和顶叶大脑皮质上方的双极硬膜外电极记录脑电图。静脉输注血清素（10微克/千克每分钟，持续10分钟）在输注后4分钟内降低了额叶和顶叶记录中的脑电图幅度。这种幅度降低是可逆的，用赛庚啶（一种有效的血清素2受体拮抗剂）预处理可防止血清素引起的脑电图幅度降低。额叶和顶叶皮质中伊文思蓝和[131I]钠的血脑屏障通透性增加。在用赛庚啶预处理的动物中，血脑屏障通透性没有这种增加。这些结果提供了直接证据，表明血液中血清素水平升高有能力影响皮质自发电活动。血清素对脑电图的这种作用似乎是由于它能够通过诱导血脑屏障的短期破坏进入脑实质，可能是通过血清素2受体。
