Härtig W, Brauer K, Fritschy J M, Brückner G, Bigl V
Department of Neurochemistry, Paul Flechsig Institute for Brain Research, University of Leipzig, Germany.
Brain Res. 1995 Sep 18;692(1-2):215-26. doi: 10.1016/0006-8993(95)00631-y.
Forebrain sections of adult male Wistar rats were processed for the immunohistochemical detection of the GABAA receptor alpha 1 subunit. Alternate sections were used for double-staining with antibodies to glutamic acid decarboxylase (GAD), choline acetyltransferase (ChAT), the calcium binding proteins parvalbumin (PARV), calbindin (CALB) and calretinin (CR) as well as to nitric oxide synthase (NOS). alpha 1 receptor subunit-immunoreactive neurons were found to be inhomogeneously distributed in the rat basal forebrain. Numerous alpha 1 subunit-immunostained neurons occupied the central part of medial septum and diagonal band, the whole ventral pallidum and the globus pallidus. A moderate number was found in the lateral septum, and only very few in the striatum and nucleus accumbens. Double-immunofluorescence labelling revealed an apparently complete co-expression of GAD-immunoreactivity in alpha 1 subunit-immunoreactive cells of rat basal forebrain, but only a region-dependent proportion of GAD-immunoreactive cells showed alpha 1 subunit-immunoreactivity. Co-expression of PARV-immunoreactivity characterized the vast majority of the alpha 1 subunit-immunoreactive cells in the medial septum, diagonal band, ventral pallidum and globus pallidus. Striatal alpha 1 subunit-immunopositive neurons appeared PARV-immunonegative and did also not react with the other immunoreagents used in this study, except the GAD-antibody. CR-immunoreactivity was co-expressed in alpha 1 subunit-immunopositive cells of the ventral lateral septal nucleus and only exceptionally in the ventral pallidum, where the vast majority of CR-positive cells was monolabelled. A small minority of ChAT-immunoreactive, but in no case CALB- and NOS-immunoreactive cells were found to express the alpha 1 subunit-immunoreactivity. These findings confirm the data obtained by analyses of other brain regions suggesting a preferred co-existence of this GABAA receptor subunit with PARV and to a lesser degree with CR.
对成年雄性Wistar大鼠的前脑切片进行处理，以免疫组织化学方法检测GABAA受体α1亚基。用抗谷氨酸脱羧酶（GAD）、胆碱乙酰转移酶（ChAT）、钙结合蛋白小白蛋白（PARV）、钙结合蛋白（CALB）和钙视网膜蛋白（CR）以及一氧化氮合酶（NOS）的抗体对交替切片进行双重染色。发现α1受体亚基免疫反应性神经元在大鼠基底前脑分布不均匀。许多α1亚基免疫染色的神经元占据内侧隔核和斜角带的中央部分、整个腹侧苍白球和苍白球。在外侧隔核中发现数量适中，而在纹状体和伏隔核中仅发现极少数。双重免疫荧光标记显示，在大鼠基底前脑的α1亚基免疫反应性细胞中，GAD免疫反应性明显完全共表达，但只有部分区域依赖比例的GAD免疫反应性细胞显示α1亚基免疫反应性。PARV免疫反应性的共表达是内侧隔核、斜角带、腹侧苍白球和苍白球中绝大多数α1亚基免疫反应性细胞的特征。纹状体中α1亚基免疫阳性神经元显示PARV免疫阴性，并且除了GAD抗体外，也不与本研究中使用的其他免疫试剂发生反应。CR免疫反应性在腹外侧隔核中的α1亚基免疫阳性细胞中共表达，仅在腹侧苍白球中偶尔共表达，在腹侧苍白球中绝大多数CR阳性细胞为单标记。发现一小部分ChAT免疫反应性细胞，但未发现CALB和NOS免疫反应性细胞表达α1亚基免疫反应性。这些发现证实了通过对其他脑区分析获得的数据，表明该GABAA受体亚基与PARV优先共存，与CR共存程度较低。
