Huang Y Y, Kandel E R, Varshavsky L, Brandon E P, Qi M, Idzerda R L, McKnight G S, Bourtchouladze R
Howard Hughes Medical Institute, Columbia University, New York, New York 10032, USA.
Cell. 1995 Dec 29;83(7):1211-22. doi: 10.1016/0092-8674(95)90146-9.
Using a genetic approach, we assessed the effects of mutations in protein kinase A (PKA) on long-term potentiation (LTP) in the mossy fiber pathway and its relationship to spatial and contextual learning. Ablation by gene targeting of the C beta 1 or the RI beta isoform of PKA produces a selective defect in mossy fiber LTP, providing genetic evidence for the role of these isoforms in the mossy fiber pathway. Despite the elimination of mossy fiber LTP, the behavioral responses to novelty, spatial learning, and conditioning to context are unaffected. Thus, contrary to current theories about hippocampal function, mossy fiber LTP does not appear to be required for spatial or contextual learning. In the absence of mossy fiber LTP, adequate spatial and contextual information might reach the CA1 region via other pathways from the entorhinal cortex.
通过遗传学方法，我们评估了蛋白激酶A（PKA）突变对苔藓纤维通路中长时程增强（LTP）的影响及其与空间和情境学习的关系。通过基因靶向敲除PKA的Cβ1或RIβ亚型会在苔藓纤维LTP中产生选择性缺陷，为这些亚型在苔藓纤维通路中的作用提供了遗传学证据。尽管苔藓纤维LTP被消除，但对新奇事物的行为反应、空间学习以及对情境的条件反射均未受影响。因此，与当前关于海马体功能的理论相反，空间或情境学习似乎并不需要苔藓纤维LTP。在没有苔藓纤维LTP的情况下，足够的空间和情境信息可能通过来自内嗅皮质的其他通路到达CA1区域。
