Kilic F, Bhardwaj R, Trevithick J R
Department of Biochemistry, University of Western Ontario, London, Canada.
Acta Ophthalmol Scand. 1996 Aug;74(4):372-8. doi: 10.1111/j.1600-0420.1996.tb00712.x.
The effect of a novel flavonoid, venoruton (a mixture of mono-, di-, tri- and tetrahydroxyethylrutosides) has been investigated in healthy rat lenses and compared with diabetic cataract modelled in vitro. One mM venoruton was added to medium simulating healthy and diabetic conditions for the incubated lenses; damage was followed by either stereoscopic photography of the lenses under a Cooperative Cataract Research Group operating microscope or with our recently developed method: the leakage of lactate dehydrogenase (LDH) into the lens culture media. The increased LDH activity in the medium and observable development of the opacity were correlated with cell damage, which has been found to be associated with globular degeneration and cataract formation. The extent of opacification and LDH release is reduced if 1 mM venoruton is included in the medium. The protective effect may be related to antioxidant activity against reactive oxygen species: decreased luminol luminescence was shown after venoruton addition to either superoxide-generating hypoxanthine plus xanthine oxidase, or hydrogen peroxide.
研究了一种新型黄酮类化合物维脑路通（单、二、三及四羟乙基芦丁糖苷的混合物）对健康大鼠晶状体的作用，并与体外模拟糖尿病性白内障的情况进行了比较。将1 mM维脑路通添加到模拟健康和糖尿病条件的晶状体孵育培养基中；通过在白内障研究协作组手术显微镜下对晶状体进行立体摄影或采用我们最近开发的方法：乳酸脱氢酶（LDH）泄漏到晶状体培养基中来监测损伤情况。培养基中LDH活性的增加以及可观察到的混浊发展与细胞损伤相关，已发现细胞损伤与球状变性和白内障形成有关。如果培养基中含有1 mM维脑路通，混浊程度和LDH释放量会降低。这种保护作用可能与针对活性氧的抗氧化活性有关：在向产生超氧化物的次黄嘌呤加黄嘌呤氧化酶或过氧化氢中添加维脑路通后，鲁米诺发光降低。
