Leese G P, Bowtell J, Mudambo S, Reynolds N, Thompson J, Srimgeour C M, Rennie M J
Department of Anatomy and Physiology, University of Dundee, UK.
Eur J Appl Physiol Occup Physiol. 1995;71(4):306-10. doi: 10.1007/BF00240409.
In an attempt to measure gastric emptying of carbohydrate solutions after exercise, we used the 13C acetate breath test to differentiate the gastric emptying of three approximately isoenergetic carbohydrate solutions (i.e. glucose, glucose polymer and sucrose) from each other and from water. On four separate occasions, six post-absorptive subjects walked on an inclined treadmill at 70% maximum oxygen uptake for 1 h and were then given 330 ml of one of the solutions in which 150 mg of sodium 1-[13C] acetate had been dissolved. Breath samples were collected at regular (2-30 min) intervals over the next 3.5 h for analysis of expired 13CO2 by isotope ratio mass spectrometry. When water was given, all subjects reached peak breath enrichment after 30 min, and had a mean (SE) gastric emptying time of 33.2 (1.6) min. Peak breath enrichment occurred later for sucrose and glucose polymer at 54.3 (3.1) min and 59.0 (2.1) min respectively (P < 0.01), and for glucose this was even later, at 62.3 (1.0) min (P < 0.05). Calculated gastric emptying times for sucrose and glucose polymer were almost identical [66.5 (2.5) and 69.8 (2.9) min respectively], whereas that for glucose was significantly slower [76.8 (3.2) min; P < 0.02], probably reflecting the effects of increased osmolality. The gastric emptying of all carbohydrates were significantly longer than for water (P < 0.01). These results show that in the post-exercise state the 13C acetate breath test can be used to differentiate the gastric emptying rates of water and carbohydrate solutions of different properties.
为了测量运动后碳水化合物溶液的胃排空情况，我们采用¹³C 乙酸呼气试验来区分三种能量大致相等的碳水化合物溶液（即葡萄糖、葡萄糖聚合物和蔗糖）之间以及它们与水的胃排空情况。在四个不同的时间段，六名吸收后状态的受试者在倾斜跑步机上以最大摄氧量的70%步行1小时，然后给予330毫升其中一种已溶解150毫克¹-[¹³C]乙酸钠的溶液。在接下来的3.5小时内，每隔一定时间（2 - 30分钟）采集呼气样本，通过同位素比率质谱法分析呼出的¹³CO₂。给予水时，所有受试者在30分钟后达到呼气富集峰值，平均（标准误）胃排空时间为33.2（1.6）分钟。蔗糖和葡萄糖聚合物的呼气富集峰值出现较晚，分别为54.3（3.1）分钟和59.0（2.1）分钟（P < 0.01），葡萄糖的呼气富集峰值更晚，为62.3（1.0）分钟（P < 0.05）。计算得出的蔗糖和葡萄糖聚合物的胃排空时间几乎相同[分别为66.5（2.5）分钟和69.8（2.9）分钟]，而葡萄糖的胃排空时间明显更慢[76.8（3.2）分钟；P < 0.02]，这可能反映了渗透压升高的影响。所有碳水化合物的胃排空时间均显著长于水（P < 0.01）。这些结果表明，在运动后状态下，¹³C 乙酸呼气试验可用于区分水和不同性质碳水化合物溶液的胃排空速率。
