脂多糖引发小鼠类过敏反应所需肽聚糖片段的结构特征。
Structural characteristics of peptidoglycan fragments required to prime mice for induction of anaphylactoid reactions by lipopolysaccharides.
作者信息
Takada H, Kawabata Y, Kawata S, Kusumoto S
机构信息
Department of Microbiology and Immunology, Kagoshima University Dental School, Japan.
出版信息
Infect Immun. 1996 Feb;64(2):657-9. doi: 10.1128/iai.64.2.657-659.1996.
Structural characteristics of peptidoglycan fragments required to prime mice for the induction of anaphylactoid reactions by Salmonella abortusequi lipopolysaccharide were examined in endotoxin-resistant C3H/HeJ mice, with special focus on the disaccharide-pentapeptide [N-acetylglucosaminyl-beta(1-4)-N-acetylmuramyl-L-alanyl-D -isoglutaminyl-meso-2,6-diaminopimelyl (DAP)-D-alanyl-D -alanine] and its smaller partial derivatives. The bacterial and synthetic muramyl tripeptides (DAP- and lysine [Lys]-type, respectively) and synthetic muramyl dipeptide primed mice for induction of anaphylactoid reactions accompanied by death within 1 h. The disaccharide-tripeptide exhibited weaker activity, and the disaccharide-tetrapeptide and muramyl tetrapeptide exhibited marginal activity. In contrast, intact peptidoglycans of various bacteria and the disaccharide-pentapeptide lacked the priming activity, although they showed adjuvant activity similar to that of the above components.
在内毒素抗性C3H/HeJ小鼠中，研究了流产马沙门氏菌脂多糖引发小鼠过敏样反应所需的肽聚糖片段的结构特征，特别关注二糖 - 五肽[N - 乙酰葡糖胺基 - β(1 - 4)-N - 乙酰胞壁酰 - L - 丙氨酰 - D - 异谷氨酰胺基 - 内消旋 - 2,6 - 二氨基庚二酸（DAP） - D - 丙氨酰 - D - 丙氨酸]及其较小的部分衍生物。细菌来源的和合成的胞壁酰三肽（分别为DAP型和赖氨酸[Lys]型）以及合成的胞壁酰二肽可引发小鼠过敏样反应，并在1小时内导致死亡。二糖 - 三肽活性较弱，二糖 - 四肽和胞壁酰四肽活性微弱。相比之下，各种细菌的完整肽聚糖和二糖 - 五肽虽然显示出与上述成分相似的佐剂活性，但缺乏引发活性。
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