Yamada A, Kataoka A, Shichijo S, Kamura T, Imai Y, Nishida T, Itoh K
Department of Immunology, Kurume University School of Medicine, Japan.
Int J Cancer. 1995 Dec 20;64(6):388-93. doi: 10.1002/ijc.2910640607.
MAGE genes encoding tumor-rejection antigens recognized by cytotoxic T lymphocytes are expressed at the mRNA level in various malignant tumors. We have investigated the expression of genes MAGE-1, -2, -3/-6 and -4a/-4b at the mRNA level in malignant and non-malignant ovarian tumors as well as in normal ovaries by reverse transcription-polymerase chain reaction. MAGE-1, -2, -3/-6 and -4a/-4b were expressed in 12, 5, 11 and 4 of 58 malignant tumors, respectively. The majority of these MAGE-mRNA-positive tumors were histologically surface-epithelial-stromal tumors, in particular serous adenocarcinomas. They mostly consisted of either advanced-stage or recurrent tumors. In contrast, neither benign tumors nor normal ovaries expressed any of the MAGE genes investigated. A 46-kDa MAGE-1 protein was identified in MAGE-1-mRNA-positive serous adenocarcinomas by immunoblot analysis with polyclonal anti-MAGE-1 antibody. These results provide important information for specific immunotherapy of ovarian serous adenocarcinomas with MAGE gene products.
编码可被细胞毒性T淋巴细胞识别的肿瘤排斥抗原的MAGE基因在多种恶性肿瘤中呈mRNA水平表达。我们通过逆转录-聚合酶链反应研究了MAGE-1、-2、-3/-6和-4a/-4b基因在恶性和非恶性卵巢肿瘤以及正常卵巢中的mRNA水平表达。MAGE-1、-2、-3/-6和-4a/-4b在58例恶性肿瘤中分别有12例、5例、11例和4例表达。这些MAGE-mRNA阳性肿瘤大多数在组织学上为表面上皮-间质肿瘤，尤其是浆液性腺癌。它们大多由晚期或复发性肿瘤组成。相比之下，良性肿瘤和正常卵巢均未表达所研究的任何MAGE基因。通过用多克隆抗MAGE-1抗体进行免疫印迹分析，在MAGE-1-mRNA阳性浆液性腺癌中鉴定出一种46 kDa的MAGE-1蛋白。这些结果为用MAGE基因产物对卵巢浆液性腺癌进行特异性免疫治疗提供了重要信息。
