嗜盐嗜碱菌基因组中根据培养基盐度差异转录的区域
Differentially transcribed regions of Haloferax volcanii genome depending on the medium salinity.
作者信息
Ferrer C, Mojica F J, Juez G, Rodríguez-Valera F
机构信息
Departamento de Genética y Microbiología, Universidad de Alicante, Spain.
出版信息
J Bacteriol. 1996 Jan;178(1):309-13. doi: 10.1128/jb.178.1.309-313.1996.
Abstract
To identify genomic regions involved in osmoregulation in the extremely halophilic archaeon Haloferax volcanii, we used a technique which involves hybridization of cDNAs obtained at different salinities against a cosmid library of the organism. Both low and high salt concentrations trigger differential expression; however, adaptation to low salinities seems to elicit a wider response. The presence of a large domain within the largest of the megaplasmids with a strong response to low salt concentrations is noteworthy.
摘要
为了鉴定极端嗜盐古菌沃氏嗜盐富球菌中参与渗透调节的基因组区域，我们使用了一种技术，该技术涉及将在不同盐度下获得的cDNA与该生物体的黏粒文库进行杂交。低盐和高盐浓度都会引发差异表达；然而，适应低盐度似乎会引发更广泛的反应。值得注意的是，最大的巨型质粒中存在一个对低盐浓度有强烈反应的大结构域。
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