Mojica F J, Juez G, Rodríguez-Valera F
Departamento de Genética Molecular y Microbiología, Universidad de Alicante, Spain.
Mol Microbiol. 1993 Aug;9(3):613-21. doi: 10.1111/j.1365-2958.1993.tb01721.x.
Two genomic sequences from the halophilic archaeon Haloferax mediterranei, where we had found PstI restriction-pattern modifications depending on the salinity of the growth medium, have been studied. A markedly salt-dependent differential expression has been detected in the nearby regions. Two of the open reading frames characterized correspond to two of the differentially expressed transcripts. In both cases the PstI sites were included in purine-pyrimidine alternancies suggestive of Z-DNA structures and located in non-coding regions with frequent repetitive motifs. A long alternating adenine-thymine tract also appears in the upstream regions of one of these open reading frames. A possible role of local DNA configuration in osmoregulation in this organism is discussed.
我们研究了嗜盐古菌地中海嗜盐嗜盐菌（Haloferax mediterranei）的两个基因组序列，在其中我们发现了取决于生长培养基盐度的PstI限制性模式修饰。在附近区域检测到明显的盐依赖性差异表达。鉴定出的两个开放阅读框对应于两个差异表达的转录本。在这两种情况下，PstI位点都包含在提示Z-DNA结构的嘌呤-嘧啶交替序列中，并且位于具有频繁重复基序的非编码区域。在这些开放阅读框之一的上游区域也出现了一个长的腺嘌呤-胸腺嘧啶交替序列。讨论了局部DNA构型在该生物体渗透调节中的可能作用。
