乙醛酸旁路操纵子阻遏物编码基因iclR的自动调节。
Autoregulation of iclR, the gene encoding the repressor of the glyoxylate bypass operon.
作者信息
Gui L, Sunnarborg A, Pan B, LaPorte D C
机构信息
Department of Biochemistry, University of Minnesota, Minneapolis 55455, USA.
出版信息
J Bacteriol. 1996 Jan;178(1):321-4. doi: 10.1128/jb.178.1.321-324.1996.
Abstract
The aceBAK operon was partially induced by a multicopy plasmid which carried the promoter region of the gene which encodes its repressor, iclR. Gel shift and DNase I analyses demonstrated that IclR binds to its own promoter. Disruption of iclR increased the expression of an iclR::lacZ operon fusion. Although aceBAK and iclR are both regulated by IclR, aceBAK expression responds to the carbon source, while expression of iclR does not.
摘要
aceBAK操纵子被携带编码其阻遏物iclR基因启动子区域的多拷贝质粒部分诱导。凝胶迁移和DNA酶I分析表明，IclR与其自身的启动子结合。iclR的破坏增加了iclR::lacZ操纵子融合体的表达。尽管aceBAK和iclR都受IclR调控，但aceBAK的表达对碳源有反应，而iclR的表达则无此反应。
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