兔出血症病毒多聚蛋白的加工过程。
Processing of rabbit hemorrhagic disease virus polyprotein.
作者信息
Martín Alonso J M, Casais R, Boga J A, Parra F
机构信息
Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, Spain.
出版信息
J Virol. 1996 Feb;70(2):1261-5. doi: 10.1128/JVI.70.2.1261-1265.1996.
Abstract
Expression of rabbit hemorrhagic disease virus (RHDV) cDNAs in vitro with rabbit reticulocyte lysates and in Escherichia coli have been used to study the proteolytic processing of RHDV polyprotein encoded by ORF1. An epitope tag was used for monitoring the gene products by a specific antibody. We have identified four gene products with molecular masses of 80, 43, 73, and 60 kDa, from the amino to the carboxy terminus of the polyprotein. The amino-terminal sequences of the 43- and 73-kDa products were determined and indicated that RHDV 3C proteinase cleaved Glu-Gly peptide bonds.
摘要
利用兔网织红细胞裂解物在体外以及在大肠杆菌中表达兔出血症病毒（RHDV）的cDNA，来研究由ORF1编码的RHDV多聚蛋白的蛋白水解加工过程。使用一个表位标签通过特异性抗体监测基因产物。我们已鉴定出从多聚蛋白的氨基端到羧基端分子量分别为80、43、73和60 kDa的四种基因产物。确定了43 kDa和73 kDa产物的氨基端序列，结果表明RHDV 3C蛋白酶切割Glu-Gly肽键。
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2
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3
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