Ivanov V A, Fel' V Ia
Tsitologiia. 1995;37(3):227-31.
A comparative study was carried out of a single carcinogenic injection of rats with a "weak" carcinogen, 4-dimethylaminoazobenzene (DAB), and a "strong" carcinogen, N-diethylnitrozamine (DENA). Both carcinogenic agents caused similar antigenic rearrangements involving the appearance of membrane hetero-organic antigens of kidney origin associated with the Zajdela hepatoma on the hepatocyte membrane. The expression of these antigens is longer in DENA carcinogenesis. On the sections of embryonic liver (16-18 days) single hepatocytes were discovered carrying membrane hetero-organic antigens. No synthesis of these antigens was discovered in cells of the definitive liver, but it was seen to resume at early stages of carcinogenesis. Quantitative characteristics of synthesis of the investigated antigens was done by means of immunocytofluorometric method using membranes of isolated hepatoma cells and hepatocytes of rats after a single carcinogenic injection and partial hepatectomy.
对大鼠单次注射“弱”致癌物4-二甲基氨基偶氮苯（DAB）和“强”致癌物N-二乙基亚硝胺（DENA）进行了一项对比研究。两种致癌剂均引起了相似的抗原重排，包括与扎伊德赫拉肝癌相关的肾源性膜异质性有机抗原出现在肝细胞膜上。这些抗原在DENA致癌过程中的表达持续时间更长。在胚胎肝切片（16 - 18天）中，发现单个肝细胞携带着膜异质性有机抗原。在成熟肝的细胞中未发现这些抗原的合成，但在致癌早期又重新出现。通过免疫细胞荧光法，利用单次致癌注射和部分肝切除后大鼠分离的肝癌细胞膜和肝细胞，对所研究抗原的合成进行了定量分析。
