[A comparative study of the synthesis of membrane hetero-organic antigens of kidney origin on tissue slices and isolated cells after a single carcinogenic exposure in rats].

A comparative study was carried out of a single carcinogenic injection of rats with a "weak" carcinogen, 4-dimethylaminoazobenzene (DAB), and a "strong" carcinogen, N-diethylnitrozamine (DENA). Both carcinogenic agents caused similar antigenic rearrangements involving the appearance of membrane hetero-organic antigens of kidney origin associated with the Zajdela hepatoma on the hepatocyte membrane. The expression of these antigens is longer in DENA carcinogenesis. On the sections of embryonic liver (16-18 days) single hepatocytes were discovered carrying membrane hetero-organic antigens. No synthesis of these antigens was discovered in cells of the definitive liver, but it was seen to resume at early stages of carcinogenesis. Quantitative characteristics of synthesis of the investigated antigens was done by means of immunocytofluorometric method using membranes of isolated hepatoma cells and hepatocytes of rats after a single carcinogenic injection and partial hepatectomy.