De Vita R, Cavallo D, Raganella L, Eleuteri P, Grollino M G, Calugi A
Environmental Department, ENEA C.R. Casaccia, Rome, Italy.
Bioelectromagnetics. 1995;16(5):330-4. doi: 10.1002/bem.2250160510.
Flow cytometry (FCM) was performed to monitor the cellular effects of extremely-low-frequency magnetic field on mouse spermatogenesis. Groups of five male hybrid F1 mice aged 8-10 weeks were exposed to 50 Hz magnetic field. The strength of the magnetic field was 1.7 mT. Exposure times of 2 and 4 h were chosen. FCM measurements were performed 7, 14, 21, 28, 35, and 42 days after treatment. For each experimental point, a sham-treated group was used as a control. The possible effects were studied by analyzing the DNA content distribution of the different cell types involved in spermatogenesis and using the elongated spermatids as the reference population. The relative frequencies of the various testicular cell types were calculated using specific software. In groups exposed for 2 h, no effects were observed. In groups exposed for 4 h, a statistically significant (P < 0.001) decrease in elongated spermatids was observed at 28 days after treatment. This change suggests a possible cytotoxic and/or cytostatic effect on differentiating spermatogonia. However, further studies are being carried out to investigate the effects of longer exposure times.
进行流式细胞术（FCM）以监测极低频磁场对小鼠精子发生的细胞效应。将每组5只8 - 10周龄的雄性杂交F1小鼠暴露于50 Hz磁场中。磁场强度为1.7 mT。选择2小时和4小时的暴露时间。在处理后7、14、21、28、35和42天进行FCM测量。对于每个实验点，使用假处理组作为对照。通过分析精子发生过程中不同细胞类型的DNA含量分布，并以伸长的精子细胞作为参考群体，研究可能的效应。使用特定软件计算各种睾丸细胞类型的相对频率。在暴露2小时的组中，未观察到效应。在暴露4小时的组中，在处理后28天观察到伸长的精子细胞有统计学显著（P < 0.001）减少。这种变化表明对分化中的精原细胞可能存在细胞毒性和/或细胞抑制作用。然而，正在进行进一步研究以调查更长暴露时间的影响。
