De Vita R, Calugi A, Chiarantano C, Forte D, Mauro F, Uccelli R
Divisione di Fisica e Scienze Biomediche, ENEA Casaccia, Rome, Italy.
Int J Hyperthermia. 1990 May-Jun;6(3):543-51. doi: 10.3109/02656739009140950.
The effects of heat on mouse spermatogenesis have been determined using both testis weight and flow cytometrically determined DNA content distribution as experimental end-points. Temperatures of 38-42 degrees C and exposure times of 20-60 min have been tested. The results concerning the testis weight substantially confirm those reported by other authors (Hand et al. 1979). The measurement of DNA content distributions shows a relatively higher depletion, 14 days after treatment, of the cytometric compartment containing elongated spermatids in respect to that containing round spermatids. The analysis of the cytotoxic effects, monitored 14 vs. 28 days after treatment, as a function of the exposure time at a given temperature, or of the temperature for a fixed exposure time, indicates that, in the course of spermatogenesis, late spermatocytes are more sensitive to heat than differentiated spermatogonia. Following the approach based on flow cytometry, the effect of exposures as low as 20 min at 38 degrees C can be appreciated.
利用睾丸重量和流式细胞仪测定的DNA含量分布作为实验终点，已确定了热对小鼠精子发生的影响。已测试了38 - 42摄氏度的温度和20 - 60分钟的暴露时间。关于睾丸重量的结果基本证实了其他作者（Hand等人，1979年）所报道的结果。DNA含量分布的测量显示，在处理后14天，相对于含有圆形精子细胞的细胞计量区室，含有伸长精子细胞的区室损耗相对较高。对处理后14天与28天监测的细胞毒性作用进行分析，作为给定温度下暴露时间或固定暴露时间下温度的函数，表明在精子发生过程中，晚期精母细胞比分化的精原细胞对热更敏感。按照基于流式细胞术的方法，可以认识到在38摄氏度下低至20分钟的暴露效应。
