Del Fiacco M, Dessì M L, Levanti M C
Neuroscience. 1984 Jun;12(2):591-611. doi: 10.1016/0306-4522(84)90075-7.
The localization of substance P-immunoreactive nervous structures in the newborn and adult human brainstem is studied by the indirect immunofluorescence technique of Coons and collaborators. Substance P-containing perikarya, nerve fibres and terminals are widely and unevenly distributed in several areas of the human brainstem, with a location similar to that described in other mammals; however, some remarkable differences are detectable, which speak in favour of the existence of species differences. For example, in the human brain there is a dense plexus of substance P-immunoreactive fibres in the cuneate fasciculus. The use of the newborn tissue is very helpful in the detection of the immunoreactive neuronal cell bodies. The comparative study of the newborn and the adult tissue reveals that the distribution of the substance P-like immunoreactivity changes in man during ontogeny and results in a more complete map of the substance P-containing neuron system.
运用库恩斯及其合作者的间接免疫荧光技术，对新生和成年人类脑干中P物质免疫反应性神经结构进行定位研究。含P物质的神经元胞体、神经纤维和终末广泛且不均匀地分布于人类脑干的多个区域，其分布位置与其他哺乳动物中所描述的相似；然而，可检测到一些显著差异，这表明存在物种差异。例如，在人类大脑中，楔束内有密集的P物质免疫反应性纤维丛。使用新生组织对于检测免疫反应性神经元胞体非常有帮助。对新生组织和成年组织的比较研究表明，在个体发育过程中，人类P物质样免疫反应性的分布发生变化，从而形成了更完整的含P物质神经元系统图谱。
