Nishimura T, Akiyama H, Yonehara S, Kondo H, Ikeda K, Kato M, Iseki E, Kosaka K
Department of Psychiatry, Yokohama City University School of Medicine, Japan.
Brain Res. 1995 Oct 16;695(2):137-45. doi: 10.1016/0006-8993(95)00699-q.
Fas antigen (CD95) is a cell surface protein that mediates apoptosis. We have investigated the immunohistochemical localization of Fas antigen in postmortem brain tissue from control subjects, patients with Alzheimer-type dementia (ATD), and from a few patients with diffuse Lewy body disease, progressive supranuclear palsy and adrenoleukodystrophy. In all brains, including controls, vascular endothelial cells and residual blood plasma were weakly stained. In ATD brains, senile plaques and a small number of star-like cells were brains of patients with neurological diseases other than ATD. In double immunostaining for Fas and glial fibrillary acidic protein (GFAP), a small number of cells were positive for both antigens. The majority of Fas-positive astrocytes were, however, negative for GFAP. This implies the downregulation of GFAP production in these cells. Doubly labeled astrocytes were also found around senile plaques, suggesting that the Fas immunoreactivity in senile plaques was derived from astrocytic membranes. The results of this study indicate that Fas antigen is expressed by a subset of reactive astrocytes in degenerative neurological diseases. Such astrocytes may undergo the Fas-mediated apoptotic process.
Fas抗原（CD95）是一种介导细胞凋亡的细胞表面蛋白。我们研究了Fas抗原在对照组、阿尔茨海默型痴呆（ATD）患者以及少数弥漫性路易体病、进行性核上性麻痹和肾上腺脑白质营养不良患者的死后脑组织中的免疫组织化学定位。在所有大脑中，包括对照组，血管内皮细胞和残留血浆呈弱阳性染色。在ATD大脑中，老年斑和少数星形细胞在非ATD的神经疾病患者大脑中也有发现。在Fas和胶质纤维酸性蛋白（GFAP）的双重免疫染色中，少数细胞两种抗原均呈阳性。然而，大多数Fas阳性星形胶质细胞GFAP呈阴性。这意味着这些细胞中GFAP产生的下调。在老年斑周围也发现了双重标记的星形胶质细胞，表明老年斑中的Fas免疫反应性来源于星形胶质细胞膜。本研究结果表明，Fas抗原在退行性神经疾病中由一部分反应性星形胶质细胞表达。此类星形胶质细胞可能会经历Fas介导的凋亡过程。
