用于检测弓形虫免疫球蛋白M抗体的Vitek免疫诊断检测法。
The Vitek immunodiagnostic assay for detection of immunoglobulin M toxoplasma antibodies.
作者信息
Candolfi E, Ramirez R, Hadju M P, Shubert C, Remington J S
机构信息
Department of Immunology and Infectious Diseases, Palo Alto Medical Foundation, CA 94301, USA.
出版信息
Clin Diagn Lab Immunol. 1994 Jul;1(4):401-5. doi: 10.1128/cdli.1.4.401-405.1994.
Abstract
We compared the Vitek immunodiagnostic assay for detection of immunoglobulin M (IgM) toxoplasma antibodies (VIDAS TXM) with an IgM enzyme-linked immunosorbent assay (ELISA) which we developed. A total of 407 serum samples from 391 adults were used for this study. We also examined 17 serial serum samples from four women who had seroconverted during gestation. We observed a sensitivity of 99.5%, a specificity of 93%, a positive predictive value of 93.6%, a negative predictive value of 99.5%, and a global agreement of 96.3%. In each case of seroconversion the VIDAS TXM became positive at the same time as did the IgM ELISA.
摘要
我们将用于检测弓形虫免疫球蛋白M（IgM）抗体的Vitek免疫诊断检测法（VIDAS TXM）与我们开发的IgM酶联免疫吸附测定法（ELISA）进行了比较。本研究共使用了来自391名成年人的407份血清样本。我们还检测了四名在妊娠期发生血清转化的女性的17份连续血清样本。我们观察到其灵敏度为99.5%，特异性为93%，阳性预测值为93.6%，阴性预测值为99.5%，总体一致性为96.3%。在每例血清转化病例中，VIDAS TXM与IgM ELISA同时呈阳性。
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