Shanmugam V, Yadav K D
Department of Chemistry, University of Gorakhpur, India.
Indian J Exp Biol. 1995 Sep;33(9):705-7.
Extracellular production of alpha-rhamnosidase [EC 3.2.1.40] from an indigeneous fungal strain of R. nigricans has been demonstrated. The enzyme has been shown to follow Michaelis-Menten kinetics using p-nitrophenyl-alpha-L-rhamnopyranosid as a substrate. The pH and the temperature optima of the enzyme have been found to be around 6.5 and 60 degrees-80 degrees C respectively.
已证明从本地黑曲霉真菌菌株中可进行胞外α-鼠李糖苷酶[EC 3.2.1.40]的生产。已表明该酶以对硝基苯基-α-L-鼠李吡喃糖苷为底物时遵循米氏动力学。已发现该酶的最适pH和温度分别约为6.5和60℃ - 80℃。