Feng S, Barthold S W, Telford S R, Fikrig E
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8031, USA.
Infect Immun. 1996 Jan;64(1):363-5. doi: 10.1128/iai.64.1.363-365.1996.
Immunization of C3H mice with P55 (previously called S1), a 55-kDa Borrelia burgdorferi antigen that is immunogenic after infection, elicited a strong antibody response but did not protect mice against B. burgdorferi challenge. Mice immunized with a P55 fusion protein in complete Freund's adjuvant developed anti-P55 antibodies, detectable at a titer of 1:10,000 by immunoblotting. To determine, if a protective response had been elicited, P55-vaccinated mice were fed upon by ticks infected with B. burgdorferi. The frequency of B. burgdorferi infection was similar in P55-immunized and control mice, and spirochetes were not destroyed within ticks that fed on P55-vaccinated mice. P55 is an immunogenic antigen that does not induce a protective response in the vertebrate or invertebrate host.
用P55（先前称为S1）免疫C3H小鼠，P55是一种55 kDa的伯氏疏螺旋体抗原，感染后具有免疫原性，可引发强烈的抗体反应，但不能保护小鼠免受伯氏疏螺旋体攻击。在完全弗氏佐剂中用P55融合蛋白免疫的小鼠产生了抗P55抗体，通过免疫印迹法可检测到其效价为1:10,000。为了确定是否引发了保护性反应，用感染了伯氏疏螺旋体的蜱叮咬接种P55的小鼠。接种P55的小鼠和对照小鼠中伯氏疏螺旋体感染的频率相似，并且在叮咬接种P55小鼠的蜱体内螺旋体未被破坏。P55是一种免疫原性抗原，在脊椎动物或无脊椎动物宿主中均不诱导保护性反应。
