Nguyen T P, Lam T T, Barthold S W, Telford S R, Flavell R A, Fikrig E
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520.
Infect Immun. 1994 May;62(5):2079-84. doi: 10.1128/iai.62.5.2079-2084.1994.
We determined whether Borrelia burgdorferi outer surface proteins (Osps) E and F could elicit immune responses useful for a Lyme disease vaccine. Thirty days after challenge with B. burgdorferi, mice produced antibodies to OspE but not OspF, whereas antibodies to OspF were present in sera of mice obtained 90 days after infection. Examination of sera from patients with Lyme disease revealed antibodies to OspF in a small number (14%) of early-stage disease patients but in a majority (58%) of patients with late-stage disease, while antibodies to OspE were rarely detected in patients. Mice immunized with recombinant OspE or OspF produced high titers of antibodies to OspE or OspF, respectively. OspF-immunized mice were partially protected from both intradermal syringe challenge and tick-mediated transmission of B. burgdorferi while vaccination with OspE did not confer immunity. B. burgdorferi organisms were, however, substantially destroyed within ticks that engorged on either OspE- (75% reduction in the number of spirochetes within the ticks, compared with controls) or OspF (90% reduction in the number of spirochetes within the ticks)-immunized mice.
我们确定了伯氏疏螺旋体外膜蛋白（Osps）E和F是否能引发对莱姆病疫苗有用的免疫反应。用伯氏疏螺旋体攻击小鼠30天后，小鼠产生了针对OspE的抗体，但未产生针对OspF的抗体，而在感染90天后获得的小鼠血清中存在针对OspF的抗体。对莱姆病患者血清的检测显示，少数（14%）早期疾病患者血清中有针对OspF的抗体，而大多数（58%）晚期疾病患者血清中有该抗体，而患者血清中很少检测到针对OspE的抗体。用重组OspE或OspF免疫的小鼠分别产生了高滴度的针对OspE或OspF的抗体。用OspF免疫的小鼠在皮内注射攻击和蜱介导的伯氏疏螺旋体传播中均得到部分保护，而用OspE疫苗接种未产生免疫力。然而，在吸食了用OspE（与对照组相比，蜱体内螺旋体数量减少75%）或OspF（蜱体内螺旋体数量减少90%）免疫的小鼠的蜱体内，伯氏疏螺旋体被大量破坏。
