Malawista S E, Barthold S W, Persing D H
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut.
J Infect Dis. 1994 Nov;170(5):1312-6. doi: 10.1093/infdis/170.5.1312.
Persistence of Borrelia burgdorferi DNA in tissues following antibiotic treatment was evaluated in C3H mice inoculated intradermally with 10(3) B. burgdorferi N40 or sterile medium. Half of the infected mice and all of the uninfected mice were treated with ceftriaxone 15 days after inoculation for 5 days. Ear and urinary bladder samples were collected on days 20, 30, and 60 after inoculation for culture and for extraction of DNA and amplification of specific spirochetal DNA by polymerase chain reaction (PCR). PCR primers were specific for a 280-bp portion of a highly conserved region of the gene encoding outer surface protein (Osp) A of B. burgdorferi and for a 328-bp part of the OspB gene. There was excellent concordance between culture and PCR for ears (35/36 mice) and bladders (33/36). Both tissues became uniformly negative at the earliest interval tested after antibiotic treatment. Thus, the ability to amplify B. burgdorferi DNA quickly disappeared from tissues that had become culture-negative after antibiotic treatment, suggesting that serial study of PCR-positive tissues and fluids may be useful for evaluating the efficacy of antibiotic therapy in human Lyme disease.
在接种10³ 伯氏疏螺旋体N40或无菌培养基的C3H小鼠中，评估抗生素治疗后组织中伯氏疏螺旋体DNA的持续性。接种后15天，对一半感染小鼠和所有未感染小鼠用头孢曲松治疗5天。在接种后第20、30和60天收集耳部和膀胱样本用于培养、DNA提取以及通过聚合酶链反应（PCR）扩增特定螺旋体DNA。PCR引物对编码伯氏疏螺旋体外膜蛋白（Osp）A的基因高度保守区域的280 bp片段以及OspB基因的328 bp部分具有特异性。耳部（35/36只小鼠）和膀胱（33/36）的培养和PCR结果具有高度一致性。在抗生素治疗后的最早测试时间点，两种组织均变为一致阴性。因此，抗生素治疗后已变为培养阴性的组织中快速扩增伯氏疏螺旋体DNA的能力消失，这表明对PCR阳性组织和液体进行系列研究可能有助于评估人类莱姆病抗生素治疗的疗效。
