Mackenzie I C, Tonetti M S
Dental Branch, University of Texas Houston Health Science Center, Houston, USA.
J Periodontol. 1995 Nov;66(11):933-43. doi: 10.1902/jop.1995.66.11.933.
The oral, oral sulcular, and junctional epithelia of the natural gingiva each possess distinct patterns of differentiation that are demonstrable both ultrastructurally and by their individual patterns of macromolecular synthesis. The supracrestal tissues reformed around oral implants structurally resemble those of natural gingiva but little is known about phenotype changes occurring in the epithelia. To investigate whether peri-implant epithelia acquire similar patterns of differentiation to those of natural gingiva, biopsies from the supracrestal regions of five oral implants were examined by immunofluorescent methods using a panel of monoclonal antibodies with specificities for individual cytokeratins and ICAM-1, macromolecules which act as markers of the three gingival epithelial phenotypes. The observed staining patterns indicated the formation of oral, oral sulcular, and junctional epithelia which were phenotypically indistinguishable from those of natural gingival epithelia. This degree of reprogramming of epithelial gene expression is a surprising observation and the potential mechanisms leading to the development of those new epithelial phenotypes are discussed in the context of what is known about the development of natural gingiva, in terms of the possible effects of inflammation, and in relation to the known connective tissue influences on epithelial differentiation.
天然牙龈的口腔上皮、龈沟上皮和结合上皮各自具有独特的分化模式，这在超微结构上以及通过它们各自的大分子合成模式都可以得到证实。围绕口腔种植体重新形成的龈上组织在结构上类似于天然牙龈组织，但对于上皮中发生的表型变化却知之甚少。为了研究种植体周围上皮是否获得与天然牙龈相似的分化模式，使用一组对单个细胞角蛋白和细胞间黏附分子-1（ICAM-1）具有特异性的单克隆抗体，通过免疫荧光方法检查了五个口腔种植体龈上区域的活检组织，细胞角蛋白和ICAM-1这些大分子可作为三种牙龈上皮表型的标志物。观察到的染色模式表明形成了口腔上皮、龈沟上皮和结合上皮，其表型与天然牙龈上皮无法区分。上皮基因表达的这种重编程程度是一个惊人的发现，并且在已知天然牙龈发育情况、炎症可能产生的影响以及已知的结缔组织对上皮分化的影响的背景下，讨论了导致这些新上皮表型形成的潜在机制。
