Byrum B R, Slemons R D
Department of Veterinary Preventive Medicine, Ohio State University, Columbus 43210, USA.
Avian Dis. 1995 Jul-Sep;39(3):622-6.
Proteolytic bacteria were readily demonstrated among the upper respiratory tract flora of poultry in two chicken flocks and two turkey flocks. Between 20% and 50% of birds in each flock had highly proteolytic bacteria in the upper respiratory tract flora, and the amount of the bacterial flora determined to be highly proteolytic in any given bird ranged from none to a majority. Highly proteolytic bacteria recovered included five species of staphylococci, as well as two gram-negative bacterial species, Flavobacterium sp. and Vibrio alginolyticus. All species of staphylococci isolated have previously been recovered from animal sources, whereas Flavobacterium sp. and V. alginolyticus are usually reported to be associated with soil and surface-water origins. The protocols used to screen for proteolytic bacteria among the flora and to assess protease activity required minimal supplies and equipment.
在两个鸡群和两个火鸡群的家禽上呼吸道菌群中很容易检测到蛋白水解细菌。每个鸡群中有20%至50%的禽类上呼吸道菌群中含有高度蛋白水解细菌，并且在任何给定禽类中被确定为高度蛋白水解的细菌菌群数量从无到多数不等。分离出的高度蛋白水解细菌包括五种葡萄球菌，以及两种革兰氏阴性细菌，即黄杆菌属和溶藻弧菌。所有分离出的葡萄球菌物种之前都曾从动物源中分离出来，而黄杆菌属和溶藻弧菌通常据报道与土壤和地表水来源有关。用于在菌群中筛选蛋白水解细菌并评估蛋白酶活性的方案所需的物资和设备最少。
